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Research Article


Effect of Adding Saccharomyces cerevisiae and/or Probiotic as Dietary Supplementation in some Biochemical Traits of Local Awassi Male Lambs


Hayder Razzaq Abed1*, Drgham Hamza AL-Zwean2

1Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Kufa, Najaf; 2Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Baghdad, Iraq.


Abstract | This study was conducted to investigate the effect of Saccharomyces cerevisiae (SC) and / or probiotic supplementation as feed additives on some blood biochemical traits (Hemoglobin concentration(Hb), Total protein(TP), Total cholesterol(TCH), Triglycerides(TG) in local Awassi male lambs. This experiment was performed in Vet. Medicine College / Kufa University. Twenty four lambs at age of 2-3 months were randomly divided into four groups (6 each) as follows: Group(G) one (Control) was fedon concentrate diet at the rate of 2% BW with wheat straw, G2 was given 5g/head of SC mixed with diet, G3 was given 1g/head /weekly of probiotic (Biolact®), G4 was given SC and probiotic (Biolact®)mixed with diet as mentioned in G2 and G3 respectively. Blood samples were taken monthly to study the traits above. Results revealed that the G2,G3 and G4 showed significantly (P<0.05) higher than the control group in Hb values.G4 showed significantly (P<0.05) higher in TP values than other groups at last experiment. While inTCH, G4 illustrated the lowest values than other groups at all periods and alternated G2 the significantly decreasing was detected in TG values than other groups at the last two months. In conclusion: the SC and probiotic combination improved the blood biochemical traits in Awassi male lambs.


Keywords | Lambs, Probiotic, Biochemical, Supplementation, Traits.


Editor | Kuldeep Dhama, Indian Veterinary Research Institute, Uttar Pradesh, India.

Received | July 31, 2018; Accepted | October 04, 2018; Published | October 18, 2018

*Correspondence | Haydet Razzaq Abed, Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Kufa, Najaf, Iraq; Email:

Citation | Abed HR, Al-Zwean DH (2018). Effect of adding Saccharomyces cerevisiae and/or probiotic as dietary supplementation in some biochemical traits of local awassi male lambs. Adv. Anim. Vet. Sci. 6(12): 537-541.


ISSN (Online) | 2307-8316; ISSN (Print) | 2309-3331

Copyright © 2018 Abed and Zwean. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.




The ruminants feeding were insufficient for a long period because it was founded on the quantity without the quality basis, this case leading to a very big gap between the animals requirements and feed availability. Therefore, any attempt that target to improve the production should be done through feeding system improvements. Today, the feed characterization according to their chemical composition, and the constitution of their different fragments, is the important one of the objectives of nutritionists when consist balancing rations that get nutrients for the microorganisms in the rumen to growth and development and then of the animal (Muniz et al., 2008).


Yeast item supplementation has a many advantages in ruminant nutrition which have been exhibited an increase in nutrient digestibility, change of the volatile fatty acids concentration created in the rumen, lessening in ruminal ammonia, and increment of ruminal microorganism populace (Chaucheyras-Durand et al., 2008). Besides, yeast culture supplementation in growing lambs can possibly enhance feed intake and growth and can substitute antibiotic agents as growth enhancing feed additive substance (Tripathi and Karim, 2011).


Numerous investigations announced that SC addition animated change in blood parameters positively, for example, yeast supplementation resulted in better iron salt absorption from the small intestine, affecting positively hemoglobin (Hb) forming processes (Dobicki et al., 2005). Neither live yeast cultures nor dried yeast significantly affected the


Table 1: Effect of dietary Saccharomyces cerevisiae and/or Probiotic supplementation in Hb of local Awassi male lambs M±SE.


Periods (monthly) Groups Zero 1 2 3 4 5 6
G1 B7.02 ± 0.54b A8.57 ± 0.59b AB7.66 ± 0.61b AB7.34 ± 0.49b AB7.32 ± 0.37b AB7.80 ± 0.61b A8.04 ± 0.54b
G2 B8.85 ± 0.37b A10.94 ± 0.21a A10.42 ± 0.22a B9.00 ± 0.27a B9.28 ± 0.22a B8.75 ± 0.49ab A9.35 ± 0.19a
G3 A11.50 ± 0.37a AB10.57 ± 0.33a BC10.02 ± 0.37a C9.06 ± 0.22a C9.10 ± 0.33a BC9.91 ± 0.35a C9.84 ± 0.46a
G4 AB10.82 ± 0.23a A11.31 ± 0.25a ABC10.42 ± 0.19a BC9.80 ± 0.18a C9.37 ± 0.64a BC9.97 ± 0.38a BC9.87 ± 0.21a
LSD 1.2074            


Means with a different small letter in the same column significantly different (P<0.05)

Means with a different capital letter in the same row significantly different (P<0.05)


blood total protein (BTP) values in calves (Dobicki et al., 2005; Kuprechtova and Illek, 2006), or neonatal dairy calves (Lesmeister et al., 2004) or early lactating dairy goats (Stella et al., 2007). Abu El-Ella and Kommonna (2013) reported that supplementation of yeast culture decreased cholesterol concentration. Also Mousa et al. (2012) worked on sheep and reported that feeding diets treated with probiotic resulted in a decrease of cholesterol concentration. Then again, the useful impacts of dietary yeast addition on the levels of the various protozoa kinds in rumen encourage favorable outcomes on metabolic activities described by increments of BTP, BUN and calcium focuses and diminishing of BTG levels in rams (Galip, 2006).


Materials and methods


Animals and Experiment Design

This experiment was performed in the animal farm of Veterinary Medicine College of Kufa University from 25 March up to 20 September 2017. Twenty four Awassi male lambs at age of 2-3 months were used with a mean body weight was 14.25 kg. The animals were randomly divided into four groups (6 each) equally (body weight was considered) and treated as following:


Group one (Control) was fed on concentrate diet at the rate of 2% body weight with wheat straw daily, Group two (SC) was fed on the same diet and was given 5g/head of S. cerevisiae daily, mixed withconcentrated diet, Group three (Pro.) was fed on the same diet and was given one sachet (1g)/head /weekly of probiotic (Biolact®) mixed with concentrated diet, Group four (SC+Pro.) was fed on the same diet and the concentrated diet was mixed S. cerevisiae as mentioned in group two and probiotic (Biolact®) as mentioned in groupthree. Water was freely offered for all groups of animals.


Biochemical and Blood Analysis

The following traits estimated by the photometric method: Hemoglobin concentration (Hb) was measured according to Coles, (1986), blood Total Protein (TP) evaluated according to Henry et al. (1974), Total Cholesterol (TCH) determination according to Tietz (1999), Triglycerides (TG) estimated according to Fossati and Prencipe, (1982).


Statistical Analysis

Data was performed using the Statistical Analysis System - version 9.1. One-way, Two ANOVA and Least significant differences post hoc test were performedto assess significant differences among means. P < 0.05 was considered statistically significant (SAS, 2010).


Results and discussion


Hemoglobin (Hb) g/dl

Results revealed that all treatment groups (G2, G3 and G4) showed significantly (P<0.05) higher the control group in Hb values in all experiment months (Table 1).


The significantly (P<0.05) higher in Hb values in treatment groups (G2, G3 and G4) than control group during experiment periods and mathematical increasing in Hb values in G4 compared with G2 and G3, it could be attributed to the effect of yeast and bacterial probiotic that resulted in better iron salt absorption from the small intestine that iron salt considered the main source of hemoglobin synthesis, also probiotics were found to produce vitamins B, affecting positively blood - cell forming processes that confirmed by (Kander, 2004) and in agreement with Sarwar et al. (2011) who found that Hb, PCV and RBC’s count were higher (P<0.05) in growing Kajli lambs fed diets containing probiotics than those without it.


Total Protein (TP) g/dl

The differences among groups within each month in TP, G4 recorded significantly (P<0.05) higher in the 4th and 6th months (6.50±0.24 and 6.92±0.15) respectively than other groups Table (2).


Table 2: Effect of dietary Saccharomyces cerevisiae and/or Probiotic supplementation in TP of local Awassi male lambs M±SE.


























G1 AB6.18±0.19a B5.70±0.12a AB6.28±0.16a AB5.84±0.13a B5.74±0.15b A5.86±0.11a A6.37±0.12ab
G2 AB5.90±0.26a B5.49±0.26a AB5.90±0.25a AB5.89±0.16a B5.50±0.15b A6.25±0.33a A6.21±0.01b
G3 AB5.92±0.22a AB5.79±0.38a A6.17±0.37a AB5.77±0.07a B5.45±0.09b AB5.80±0.10a A6.53±0.14ab
G4 C5.75±0.15a B6.01±0.32a A6.35±0.19a A6.35±0.12a A6.50±0.24a AB6.27±0.18a A6.92±0.15a
LSD 0.5967            


Means with a different small letter in the same column significantly different (P<0.05)

Means with a different capital letter in the same row significantly different (P<0.05)


Table 3: Effect of dietary Saccharomyces cerevisiae and/or Probiotic supplementation in blood total Cholesterol of local Awassi male lambs M±SE.


Periods (monthly) Groups Zero 1 2 3 4 5 6




























































LSD 3.2509            


Means with a different small letter in the same column significantly different (P<0.05)

Means with a different capital letter in the same row significantly different (P<0.05)


The significant (P<0.05) increasing of TP values in group that be supplemented by yeast and bacterial probiotic (G4) than other groups during the experiment periodsit may be related to the synergism effect of probiotics (yeast + bacteria) supplementation on protein digestibility through the enzymatic effect of protease and alteration amino acid profile of digestion due to increasing microbial protein synthesis leading to increase in protein formation in the cells of liver and consequently increasing in the blood TP (Abdel-Khalek et al., 2000).


Total Cholesterol (mg/dl)

The results showed significant (P<0.05) differences among groups at different periods Table 3. The G1 recorded significantly (P<0.05) increasing in Cholesterol values compared with other groups for all experiment months which showed (G2,G3 and G4) significantly decreasing in cholesterol values during all experiment months than control group.


The significant (P<0.05) reduction in the total cholesterol level in G2,G3 and G4 as a result of SC and/or probiotic supplementation compared with control group of each experiment period, which it may be considered as an indication of the improvement in the health status of animal because of yeast and probiotic addition leading to more using of cholesterol molecule for body growth and development which confirmed by Abu El-Ella & Kommonna, (2013) who reported that supplementation of probiotic decreased cholesterol concentration and in agreement with El-Ashry et al. (2004) and Talha et al. (2009) worked on buffalo claves and Abdel Rahman et al. (2012) and Mousa et al., (2012) worked on sheep and reported that feeding diets treated with probiotic resulted in a decrease of cholesterol concentration and improve animal health. Or it may be attributed to, the enzymatic de-conjugation of bile acids (bile acids consist of cholesterol) by probiotics hydrolase (Begley et al., 2006) and once de-conjugated, bile acids became less soluble and less absorbed by the intestines, leading to their elimination in the feces.


Triglycerides (TG) mg/dl

Results demonstrated that G2 alternated G4 the significant decreasing than other groups in blood TG values which be evident during 5th and 6th months (22.89 ± 1.28 and 32.69 ± 1.40) respectively of experiment Table (4).


The significant (P<0.05) reduction in the TG values in G2 and G4 than other groups at 5th and 6th months respectively of experiment, it could be due to, the effect of yeast and


Table 4: Effect of dietary Saccharomyces cerevisiae and/or Probiotic supplementation in blood TG of local Awassi male lambs M±SE.


























G1 C18.98 ± 1.45a C19.95 ± 0.51a BC22.36 ± 1.03a

B24.58 ±


B26.03 ± 1.31a B24.75 ± 0.65ab A36.14 ± 1.18ab


± 1.05a

B20.16 ± 0.43a B22.57 ± 1.70a

B23.40 ±


B22.88 ± 0.57a

B22.89 ±


A37.45 ± 1.12a
G3 D20.54 ± 0.40a D20.85 ± 0.37a CD22.86 ± 1.62a

B26.50 ±


BC25.24 ± 2.07a BC25.22 ± 0.85ab A35.89 ± 1.49ab
G4 CD20.70 ± 0.52a D19.92 ± 0.31a CD23.92 ± 1.76a BC25.17 ± 1.61a BC25.37 ± 1.49a

B26.81 ±


A32.69 ± 1.40b
LSD 3.6126            


Means with a different small letter in the same column significantly different (P<0.05)

Means with a different capital letter in the same row significantly different (P<0.05)


probiotic supplementation leading to decrease the TG values in blood serum of supplemented animals as a result of positive changes in rumen fermentation and increase in bacterial and protozoal numbers and some changes in short-chain fatty acids concentration in the rumen that all this changes leading to reduction in TG formation in the cells of liver and consequently decreasing in the blood TG that consistent with Masek et al. (2008) and in agreement with Chiofalo et al. (2004) who reported a significant reduced in the concentration of non-esterified fatty acids (NEFA), triglycerides and increase of high density lipoproteins (HDL) in growing kids supplemented with probiotics.




Authors would like to thank the staff of the farm of faculty of Veterinary Medicine and the Public Health lab for their contribution.


Conflict of Interest


Authors declare that there was no conflict of interest.


Authors Contribution


Dr Hayder and Dr Drgham did the experimental design. Dr Hayder did the experimental work and lab work. Dr Hayder and Dr Drgham wrote the manuscript.




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