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The Journal of Advances in Parasitology

Research Article
J. Adv. Parasitol. 6(1): 7-11
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Ismail Qureshi1, Mustafa Solangi2, Ahad Soomro3, Waseem Baloch1, Sundesh Kumar1, Shahjahan Qaim Khani1

1Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, Sindh-Pakistan; 2Vaccine Production Unit (VPU) Tandojam, Sindh-Pakistan, 3Central Veterinary Diagnostic Laboratory Research and Diagnosis (CVDL) Tandojam, Sindh-Pakistan.

Abstract | To establish the baseline and the data on samples of Cutaneous Leishmaniasis were collected from three different districts of Sindh-Province, Pakistan to diagnose Leishmania species (spp), parasites through Polymerase Chain Reaction (PCR). Total, 70 human Leishmaniasis tissues from various villages of district, Dadu, Jamshoro and Hyderabad were symptomatically identified. For further confirmation all identified tissue samples were subjected to Polymerase Chain Reaction (PCR) using, Applied Bio-system thermal cycler. DNA from samples was extracted, quantified, through, Nano drop spectrophotometer and, Polymerase Chain Reaction (PCR) product was run on, Gel electrophoresis and bands were analyzed using, Gel documentation system out of, 70 suspected, Cutaneous Leishmaniasis cases, 61 were diagnosed clinically cases and confirmed by, Polymerase Chain Reaction (PCR) for, Cutaneous Leishmaniasis. During this study, both dry and wet types of lesions were observed that, indicated the presence of two strain of parasite. Children and adults between 08-60 years of ages were found more susceptible to infection when compared with above 60 years of ages. The strains were found to be Leishmania tropica and Leishmania major as was indicated by the nature of lesions and confirmed by Polymerase Chain Reaction (PCR). Cutaneous Leishmaniasis is endemic and increasing in the rural areas of Sindh province, Pakistan. This requires attention of health authorities to take appropriate measures for its effective control, failing which it may create more severe public health problems.
Keywords | Cutaneous Leishmaniasis, Sand fly, Leishmania tropica, Leishmania major, PCR, Pakistan.