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The Journal of Advances in Parasitology

Research Article
J. Adv. Parasitol. 6(1): 1-6
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Salma Mohamed Abdel-Rahman, Hanaa Yousef Bakir, Ragaa Ali Othman, Mervat Moustafa Khalifa*

Medical Parasitology Department, Faculty of Medicine, Assiut University, Assiut, Egypt.

Abstract | Human intestinal capillariasisis an emerging zoonotic parasitic disease caused by tiny nematode, Capillaria philippinensis. It is now considered an important public health problem in non-endemic areas including Egypt. Death may occur in cases of misdiagnosis and improper treatment. Yet, diagnosis is difficult due to its confused presentation with other etiologies of enteropathy and unavailability of C. philippinensis antigen. Although different types of antigens have been used for serodiagnosis of capillariasis but lack of specificity and cross reactivity is still a challenge. Therefore, in the present study we evaluate Capillaria spp. Adult worm crude antigen extracted from freshwater fish in serodiagnosis of human capillariasis using enzyme-linked immunosorbent assay (ELISA). For this reason, seven species of fresh water fish were examined for the presence of Capillaria spp. natural infection. Capillaria spp. adult and larvae were recovered from the intestine of two species of the examined fishes, bayad (bagrus bajad) and small sized bolty (Oreochromis niloticus).This antigen was able to detect anti-Capillaria antibodies in the sera of infected cases at the same OD value obtained with C. philippinensis adult worm crude antigen However, Fish Capillaria spp. adult worm antigen showed no reactivity with any of the healthy control sera. This assay highlights the usefulness of fish Capillaria antigen in diagnosis of human intestinal capillariasis.

Keywords | Capillaria philippinensis, Serodiagnosis, Fish, Bagrus bajad. Oreochromis niloticus. ELISA