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The Journal of Advances in Parasitology

Short Communication
J. Adv. Parasitol. 5(2): 25-28
Http://dx.doi.org/10.17582/journal.jap/2018/5.2.25.28
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Suleman Shah1, Haroon Akbar1, Nisar Ahmad1, Raheela Akhtar2*, Wasim Shehzad3, Saher Islam3

1Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, Pakistan. 54000; 2Department of Pathology, University of Veterinary and Animal Sciences, Lahore, Pakistan. 54000; 3Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan.

Abstract | The present study was designed to provide the information about molecular detection of Toxocara species in dogs of Pakistan for the first time. One hundred fecal samples from suspected dogs were collected from Pet Centre, University of Veterinary and Animal Sciences, Lahore and screened for Toxocara eggs through microscopy. The microscopically positive samples were further subjected to Polymerase chain reaction (PCR) targeting internal transcribed spacer region of Toxocara genome. The PCR positive samples were further purified and sequenced in both directions using Sanger sequencing method. The results indicated three samples (1.5%) positive for Toxocara through fecal microscopy while sequencing confirmed these entire samples positive for T. leonina. This study precisely diagnosed Toxocara leonine for the first time in dogs to nullify the chance of misdiagnosis by conventional fecal microscopy.

Keywords | Dog, Polymerase chain reaction, Sequencing, Toxocara leonina