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The Journal of Advances in Parasitology

Research Article
J. Adv. Parasitol. 5(2): 22-24
Http://dx.doi.org/10.17582/journal.jap/2018/5.2.22.24
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Muhammad Younus Khan1, Haroon Akbar1, Raheela Akhtar2*, Wasim Shehzad3, Saher Islam3, Beenish Zahid4

1Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, Pakistan.54000; 2Department of Pathology, University of Veterinary and Animal Sciences, Lahore, Pakistan.54000; 3Institute of Biochemistry and Biotechnology (IBBt), University of Veterinary and Animal Sciences, Lahore, Pakistan.54000; 4Department of Zoology, University of the Punjab, Pakistan.

Abstract | The aim of the present study was to optimize the polymerase chain reaction for molecular detection of Babesia felis in domestic cats. One hundred cats suspected for babesiosis with severe signs of pale mucus membrane, haemolytic anaemia, rough body coat and haemoglobinuria were recruited in this study. Polymerase chain reaction was optimised targeting amplification of 18S rRNA gene. Amplification and phylogenetic analysis confirmed Babesia felis in 14% of domestic cats in comparison to microscopy that detected only 6% of cases. PCR also indicated the higher prevalence in local breeds (16.6%) as compares to Persian (13.33%) and Siamese (11.11%). The results indicated that younger (under one year) and older (above three years) cats were more susceptible to infection. There was an increased percentage of Babesia felis in female cats (15%) as compared to male (12.5%). The application of PCR may be recommended for reliable field diagnosis of Babesia felis.

Keywords | Babesia felis, Persian, PCR, Sequencing, Siamese