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Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 6(5): 201-206
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Isa Swadi Touhali

Department of Microbiology, College of veterinary Medicine, Wasit University, Iraq.

Back ground: Cryptococcosis is the most common systemic fungal infection in humans and animals which are easily isolated from avian excreta. Therefore, it’s important to isolate them locally and to know their effect on public health by injecting them empirically into laboratory mice. Methodology: One hundred and fifty samples of pigeon droppings are collect from Wasit province, during January 2018 to March 2018.Twenty-four male adult mice type BALB/c is use, divided into two groups of 12 animals per group. One group which is consider as control, whereas other group is injected with suspension of Cryptococcus neoformans isolated from pigeon droppings as treatment group. Four animals from each group were killed every 3 days after injection serially (T1=3 days, T2=6 days, and T3=9 days respectively) to investigate the effects of C.neoformans for three injection periods on some hematological (RBC, WBC, PCV, Hb concentration, and differential WBC count), biochemical (serum ALT and AST activities) and histopathological parameters. Results: The result show twenty six droppings pigeon samples (17.3%) out of 150 samples were positive for the incidence of C.neoformans isolates and significant reduction of RBC, PCV ratio, and Hb concentration in all treated groups. While, WBC and differential WBC counts (nutrophils, monocyte and acidophil ratio) was significantly increased, while the lymphocyte and basophil ratio was reduced during the three periods of injection as compared to control. Also, the yeast suspension produced a significant elevation in the activities of serum ALT and AST with control group .but, the level of serum AST persist elevated with long injection period for 9 days than elevation of ALT comparison. As well as, data showed pathological alterations in organs (brain and lung) of mice. Conclusion: of present investigation indicates that prolong exposure of C.neoformans could mostly induced physiological alterations in blood picture, serum biochemistry and caused histopathological of brain and lung in Cryptococcus infected animals.

Keywords | Cryptococcus neoformans, Pigeon droppings, BALB/c mice