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Advances in Animal and Veterinary Sciences

Short Communication
Adv. Anim. Vet. Sci. 1 (1S): 9 - 10 Special issue-1 (Veterinarians approaches for safeguarding animal health and production)
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Ashish Kumar, Umesh Singh, Sushil Kumar, Gyanendra Sengar, Rani Singh, Rupali Singh, Arjava Sharma, Rajib Deb*
Molecular Genetics Laboratory; Project Directorate on Cattle; (Indian council of Agricultural Research); Meerut Cantt, Meerut; Uttar Pradesh––250 001, India
*Corresponding author: drrajibdeb@gmail.com

ABSTRACT
Pathogenic Escherichia coli, when colonize the bovine mammary glands, results in the cell damage as well as changes in the composition of the milk. A total 50 milk samples of Frieswal (HF x Sahiwal) cow herd maintained at Military Farm, Meerut were collected from mastitis (n= 35) and normal (n=15). Escherichia coli were detected through simplex PCR using published primers of 23s rRNA. All the 35 mastitis milk samples were positive for Escherichia coli, which was further confirmed by sequencing and phylogenetic analysis by using MEGA 5.2 software.The Phylogenetic analysis of our isolate (KF278640) with otherEscherichia coli fall in a clade with (AM158281.1) Escherichia coli 16S rRNAtRNA– Glu and 23S rRNA.

Key Words: PCR; Mastitis; Escherichia coli; Frieswal