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Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 6(3): 113-120
 
Figure 1

Milk medium showing the classical stormy clot reaction of C. perfringens.The tube on the left extreme is uninoculated. 

Figure 2

Lecithinase activity of C. perfringens in egg yolk agar after 24 hours of growth. Streaks showing opalescence production around C. perfringens. 

Figure 3

Agarose gel electrophoresis showing 775 bp amplicon of alpha toxin gene of C. perfringens. Lane M- 100 bp DNA ladder, Lane 1- Negative control, Lane 2- Positive control, Lane 3-6 – Isolates positive for C. perfringens. 

Figure 4

Intestinal mucosa showing diffuse necrosis and pseudomembrane which forming a dirty turkey towel appearance.