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Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 6(2): 70-74
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Asinamai Athliamai Bitrus2,5, Yusuf Abba2,4, Faez Firdaus Abdullah Jesse1,3*, Joy Lee Xing Pei1, Innocent Damudu Peter1,4, Idris Umar Hambali1,4, Abd Wahid Haron1, Mohd Azmi Mohd Lila1, Abdul Aziz Saharee1

1Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; 2Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; 3Institute of Tropical Agriculture and Food security, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; 4Faculty of Veterinary Medicine, University of Maiduguri. P.M.B 1069 Maiduguri, Borno Nigeria; 5Department of Veterinary Public heath, Faculty of Veterinary Science, Chulalongkorn University, 10330 Pathumwan, Bangkok.

Abstract | Bovine anaplasmosis also known as Red water or Gall sickness is an important disease of cattle primarily caused by Anaplasma marginale and it infects erythrocytes, which results to erythrophagocytosis and subsequently anaemia. This study was carried out to determine the seroprevalence of bovine anaplasmosis among dairy cattle in some randomly selected ruminant farms in Peninsular Malaysia. A total of 45 Blood samples were collected via jugular venipuncture from cattle from four (4) farms using convenient sampling technique. Twelve(12) cows were sampled from each of farms A and B, while 13 cows were sampled from farm C and 8 from Farm D. Heparinized whole blood was used to prepare Giemsa-stained thin blood smears for microscopic detection of anaplasmosis. Serum was extracted from coagulated blood for serological testing using Anaplasma antibody Test Kit (VMRD, Inc. United State of America). The result showed an overall seroprevalence rate of 51.11% (23/45). Farm level seropositivity showed 83.3% (10/12), 41.7% (5/12), 23.1% (3/13) and 62.5% (5/8) for Farms A, B, C, and D, respectively. Age specific seroprevalence showed a 53.13% (17/32) in cows that are more than 3 years old and 46.15% (6/13) in cows aged between 1-3 years. Additionally, a significant (p<0.05) association between microscopic detection and serological detection method was observed. . In conclusion, a high seroprevalence rate was observed in the selected ruminant farms.

Keywords | Bovine anaplasmosis, Anaplasmamarginale, Seroprevalence, cELISA, Microscopic detection.