Advances in Animal and Veterinary Sciences

Case Report
Adv. Anim. Vet. Sci. 5(10): 405-409
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Rahul Singh1*, Swati Kumari1, Jay Prakash Yadav2, Shivvaran Singh3, Ashok Kumar4, Susil Kumar5

1Division of Pathology, ICAR- Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122 (U.P), India; 2Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar (U.P.)-243122, India; 3Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar (U.P.)-243122, India; 4Division of Virology, ICAR-Indian Veterinary Research Institute, Izatnagar (U.P.)-243122, India; 5Division of Animal Genetic and Breeding, ICAR-Indian Veterinary Research Institute, Izatnagar (U.P.)-243122, India.

Abstract | A case of suppurative pneumonia with inflammation of lung-associated lymph node (mediastinal and bronchial) caused by Corynebacterium pseudotuberculosis was described in a 2-year-old Shirohi breed, the female goat which presented clinical sign, respiratory distress, nasal discharge with fever followed by sudden death. The suppurative inflammation was detected in lung parenchyma and lymph node was characterized by formation of caseo-calcified nodules of multiple abscesses which were scattered throughout lung lobes and even in the chest wall. The prominent enlargement of mediastinal and bronchial lymphnodes were sectionedand analysis revealed laminated calcified granules and semi-solid fluid with greenish yellow inspissated pus giving the spherical onion-skin appearance (pathognomonic signs) respectively. Histopathologically,the lung section was characterized by a central caseo-necrotic core admixed bacterial colonies and infiltration of polymorpho nuclear cells, few mononuclear cells, lymphocytes, plasma cell and macrophages. The bacterial culture examination showed the presence of bacterial growth forming cream white, dry, waxy colonies with a narrow zone of β-haemolysis.  They were stained gram positive cocco-bacilli arranged in Chinese pattern. The extracted DNA from tissue was further confirmed by polymerase chain reaction (PCR) assay for amplified pld gene specific for goat. Present casestudy detail described the pathognomonic gross, histopathological lesions ofC. pseudotuberculosis in a goat with bacteria isolation and finally confirmed by PCR.

Keywords | Suppurative pneumonia, PCR, Histopathology, Lymphadenitis, Goat