E. coli colonies with characteristic (A): Pink colour, lactose fermenting colonies on MacConkey agar (B) greenish metallic sheen on Eosin methylene blue (EMB) agar 

Gram staining showing gram negative E.coli bacilli
 

Gel image showing amplified product of E.coli virulence gene
Lane 1 : Molecular Marker of 100 bp; Lane 2: iss +ve control; Lane 3: iss representative sample; Lane 4: papC +ve control; Lane 5: papC representative sample; Lane 6:tsh +ve control; Lane 7: tsh representative sample; Lane 8: vat +ve control; Lane 9 : vat representative sample 

Graph showing virulence genes profile of thirty broiler flocks positive for Avian Pathogenic E.coli 

Plate showing in-vitro Antibiotic Susceptibility Test for E.coli isolates 

Graph showing percentage of in-vitro Antibiotic Susceptibilityof the E. coli isolates