Advances in Animal and Veterinary Sciences

Short Communication
Adv. Anim. Vet. Sci. 1 (4): 123 - 126
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Thangavel Geetha, Subodh Kumar*, Prem Prakash Dubey, Balasubramaniam Sivamani, Arjava Sharma
Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243 122, India
*Corresponding author: subkum@gmail.com

ABSTRACT
Nucleotide variability in exon 2 of CatSper1 gene (434 bp) was evaluated in crossbred cattle named Vrindavani (n = 100) using PCR–RFLP and nucleotide sequencing. Using DNA from blood, restriction enzyme analysis with EcoRI and HindIII produced two bands of 315 bp, 119 bp and 360 bp, 74 bp respectively. This amplicon of CatSper1 gene exhibited absence of polymorphism with respect to restriction enzymes used and accordingly, the allelic frequency was found to be unity. The monomorphic pattern of the amplicon of CatSper1 gene with respect to different enzymes indicated the conservedness of this gene. The amplicon was subjected to DNA sequencing, annotation and submitted to GenBank. In order to study the variation at nucleotide level, the sequence of this amplicon was compared with similar sequences of mithun, goat and buffalo and nucleotide variations ascertained.

Key Words: Catsper1, Crossbred cattle; Exon 2; EcoRI; HindIII; Vrindavani