Advances in Animal and Veterinary Sciences
Research Article
Adv. Anim. Vet. Sci. 5(4): 179-184
Http://dx.doi.org/10.17582/journal.aavs/2017/5.4.179.184
Hanaa Mohamed Ghanem, Ahmed Ibrahim Ateya, Rasha Mohamed Saleh, Mamdouh Saleh Hussein
Department of Animal Husbandry and Wealth Development, Faculty of Veterinary Medicine, Mansoura University, 35516, Egypt; 2Department of Physiology, Faculty of Veterinary Medicine, Mansoura University, Egypt; 3Department of Theriogenology, Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Mansoura University, Egypt.Abstract | The aim of this study was to investigate the impact of artificial insemination (AI) versus natural mating and genetic PRL/PstI locus polymorphism on different duck productive and reproductive aspects. The birds were divided into two groups; 5000 female for each group and the data (body weight of birds, hatchability%, fertility %, mortality %, hatching egg % and birth weight of day old chick) were analyzed using PROC MIXED procedure of SAS V9. Concerning PRL/PstI locus polymorphism, blood samples were collected from the experimental birds for DNA extraction and PCR-PstI digestion for a fragment of exon 5 (400-bp) of PRL gene was carried out. AI breeding group had significantly higher productive and reproductive parameters (average egg number, birth weight of day-old chick, fertility and hatchability %) compared to the natural mating group except for mortality %. PCR-PstI of 400-bp of PRL gene revealed two fragments (254 and 146-bp) for genotype TT, three fragments (400, 254 and 146-bp) for genotype TC and undigested fragment (400-bp) for genotype CC. The χ2-test showed that the genotype distributions in the duck population were in Hardy–Weinberg equilibrium (P<0.05). Statistical analysis revealed a significant association between PRL genotypes and the studied traits; where CC genotype was higher than both TC and TT genotypes in these traits. This research highlights the artificial insemination, natural mating, and PRL/PstI locus as candidates for different duck productive and reproductive traits.
Keywords | Duck, Artificial insemination, Natural mating, PRL gene, Productive, Reproductive traits