Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 1 (4): 116 - 119
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Gopi Muthusamy, Siddhartha Narayan Joardar*, Indranil Samanta, Devi Prasad Isore, Barun Roy1, Tapas Kumar Maiti2
Department of Veterinary Microbiology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata–700037; 1Department of Animal Nutrition, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata–700037; 2Department of Biotechnology, Indian Institute of Technology, Kharagpur–721302
*Corresponding author: joardar69@gamil.com, Fax: +91–33–25571986

 

ABSTRACT
The study was undertaken to assess the effect of orally administered β–glucan on intestinal intra epithelial leukocytes (iIEL) of broiler to establish its role in mucosal immunity. After acclimatization for 7 days with control diet, two groups of broiler birds each containing 20 chicks, with replica of 10 chicks in each pen, were fed with experimental feed containing β–glucan @15mg/ kg (group A) and 30 mg / kg (group B) for a period of 20 days and, then given normal diet. Birds kept with normal feeding regime (n=10) were considered as control (group C). Intestinal intraepithelial leucocytes (iIEL) of the experimental birds were isolated and in–vitro immune–effectors activities were observed. Significant enhancement (P ≤ 0.05) of in vitro phagocytic activity, nitrite production, proliferative activity and cytokine production of iIEL were observed in birds of group B than C. The results showed that supplementation of β–glucan from edible mushroom in feed (30mg/ kg) for 20 days had significant immunostimulatory effect in broiler birds that was reflected in the in vitro immune–effector activities of the mucosal cells (iIEL).

Key Words: Broiler, β–glucan; intestinal intraepithelial leucocytes; mucosal immunity; mushroom