Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 1 (4): 111 - 115
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Yashpal Singh Malik1, Naveen Kumar1, Kuldeep Sharma1, Rajeev Sharma2, Harsh Bardhan Kumar2, Kusumakar Anupamlal2, Savita Kumari3, Sanjay Shukla4, KM Chandrashekar2
1Indian Veterinary Research Institute, Izatnagar 243122, Uttar Pradesh, India; 2JN Krishi Vishwa Vidyalaya, Jabalpur 482001, Madhya Pradesh, India; 3Department of Microbiology, Bihar Veterinary College, Patna, India; 4College of Veterinary Science and Animal Husbandry, Madhya Pradesh Pashu Chikitsha Vigyan Viswavidyalaya, Rewa, M.P., India
*Corresponding author: malikyps@ivri.res.in

ABSTRACT
Virus induced enteritis is one of the grave problems accounting for maximum deaths in neonatal animals and human throughout the world. In developing countries like India, the socio–economic conditions favors the occurrence of higher disease frequency of gastro–enteric infections, majorly dominated by rotavirus (RV). The dearth of appropriate surveillance programs and laboratory facilities have resulted in scarcity of accessible data on RV associated enteric disease affliction and epidemiological evidence in the country. We describe here the epidemiological and genotypic distribution of RV in various animal species and human during 2004 to 2008 from Madhya Pradesh, the central region of India. The overall prevalence rate of RV was 17.19% (126/733), maximum in humans (30.23%), followed by buffaloes (22.01%), cattle (13.33%), swine (13.04%) and poultry (6.47%). The nested–polymerase chain reaction (PCR) based genotyping results revealed circulation of genotypes of G10 and G3 specificity and P[1] and P[3] types in the region. In humans, only the G1 genotype was detected and for the first time group B rotavirus was detected in an adult. Based on the typical migration pattern of RNA genome segments in the gel electrophoresis (5:2:2:2), group D rotavirus was also identified in poultry diarrheic samples. The results emphasize the need for extended molecular surveys for understanding the interspecies transmission, evolution and especially in designing future vaccine.

Key Words: Rotavirus; Epidemiology; Bovine; Porcine; Poultry; Humans; Genotyping; Madhya Pradesh; Central India