Abstract | This study was aimed to evaluate the influence of injection of hatching eggs with inactivated Newcastle disease vaccine (ND) and two doses of Cod liver oil (CLO) on hatchability, some biochemical, productive traits and immune response of broiler chickens. Two hundred fertilized eggs of broiler breeders Ross 308 strain were incubated in automatic incubator machine. They were divided into four groups (50 eggs per group) with two replicates. The first group was injected in amniotic fluid with 0.1ml of Phosphate Buffer Saline (PBS) /egg which was considered as control, the second group was injected with 0.1ml of inactivated oil emulsified ND vaccine /egg, the third group was injected with 0.1ml of inactivated oil emulsified ND vaccine and 0.1 ml of CLO /egg, the fourth group was injected with 0.1ml of inactivated oil emulsified ND vaccine and 0.15ml of CLO/egg. The eggs were returned to incubator till hatching date. Hatched chicks were reared in the hall of the Veterinary Medicine College/University of Baghdad after distributed into four groups with two replicates according to the previously treated groups until the fifth week of age. The results showed a significant (P ≤ 0.05) increased of the treated groups in hatchability, body weight and improvement in feed conversion ratio as compared with second group and control, also fourth group showed significantly increased (P ≤ 0.05) in antibody titers against ND virus at age of 21 and 35 day as compared with the other treated groups and control.
Keywords | In ovo injection, Cod liver oil, Immune response, Newcastle, Broiler