Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 1 (4): 107 - 110
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SatyaVeer Singh Malik, Durga Prasad Das*, DeepakBhiwa Rawool, Ashok Kumar, Rahul D. Suryawanshi, Mamta Negi, Jess Vergis, Swapnil Doijad2, Sukhdeo Baliram Barbuddhe2
1Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, U.P., India–243 122; 2ICAR Research Complex for Goa, Ela, Old Goa, India–403402
*Corresponding author: drdurga.prasad.das@gmail.com

 

ABSTRACT
The epidemiology of Q–fever with regard to the role of foods of animal origin in transmission of Coxiella burnetii and their risk assessment remains largely unknown in most parts of the world including India. In the present study, a total of 591 food samples of different animal origin including milk (518), meat (60) and shell eggs (13) were screened for the presence of the pathogen by trans–PCR assay targeting transposon like element IS1111 of C. burnetii. . The analysis revealed an overall positivity in 4.06% (24/591) food samples, all of which were of milk. The milk samples showed an overall positivity of 4.63% (24/518), with the detection of C. burnetii in 5.55% (23/414) bovine and 1.42% (1/70) ovine milk samples. However, all the samples of camel milk (n = 34), meat (n = 60) and shell eggs (n = 13) turned out to be negative for the pathogen. The detection of C. burnetii in the bovine and ovine milk indicates a potential health risk for domestic livestock as well as human beings, especially those who consume raw or unpasteurized milk. The study of screening foods of animal origin for occurrence of C. burnetii in India by highly sensitive and specific molecular diagnostic tools is largely lacking, and in this context, the present study appears to be the first of its kind in the country. It is recommended that milk should be consumed only after pasteurization and, dairy as well as other foods of animal origin should be included in surveillance and monitoring programmes for food–borne pathogens for the risk assessment of C. burnetii infection.

Key Words: Animal origin;Food; Coxiella burnetii; Trans PCR; India