Pear shaped piroplasm inside RBC (x100) 

PCR products amplified using bab-F and bab-R specific primers: Lane M) 100 bp DNA ladder (Invitrogen); Lane N) Negative control; Lane 2, 3 and 6) Negative samples; Lane 4, 5, 7, 8 and 9) Positive samples

Prevalence of Babesia spp. At different sampling sites 

PCR products amplified using 455-479F and 793-772R specific primers: Lane M) 100 bp DNA ladder (Invitrogen); Lane N) Negative control; Lane 2, 3, 5, 6 and 7) Negative samples; Lane 4) Positive samples 

PCR products amplified using Bgib Asia-F and 793-772R specific primers: Lane M) 100 bp DNA ladder (Invitrogen); Lane N) Negative control; Lane 2, 3, 4, 5 and 6) Negative samples; Lane 7, 8 and 9) Positive samples 

Phylogentic tree for the partial 18S rRNA gene (KR349267) from selected Babesia spp.