Advances in Animal and Veterinary Sciences

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Adv. Anim. Vet. Sci. 1 (4): 102 - 106
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Subodh Kumar*, Subuhi Khan, Ayyar Sivakumar, Hirenallur Maheshwarrappa Yathish, Balasubramaniam Sivamani, Deepak Sharma, Arjava Sharma
Division of Animal Genetics, IVRI, Izatnagar, Bareilly, Uttar Pradesh, 243 122, India
*Corresponding author:

It has been demonstrated that when the zona pellucida of hamster ova was freed, it permitted the entry of spermatozoa from hamster (homologous species) as well as spermatozoa from a variety of species (heterologous species). Suggestions have therefore been made that zona free eggs of golden syrian hamsters (Mesocricetus auratus) may provide a viable alternative to test the penetrability of spermatozoa since they allow entry of capacitated spermatozoa from a wide variety of foreign (heterologous) species. The parameters viz. sperm attached per ovum, ova attached, fertilization percent, fertilization index and polyspermic ova are evaluated in this test. Many workers have used this simple test in laboratory to evaluate the fertility potential of bull under in vitro conditions. This test have also been used in human to test the penetrability of spermatozoa of the suspected sub fertile man. We developed a simple regression equation at our lab for crossbred cattle and buffalo bulls and found a high correlation between predicted and actual fertility status. This bioassay has proved to have a high association with the fertility status of the livestock species and hence could serve as a useful tool in identifying potentially sub fertile males before they enter into the sire sampling programme. This would tend to make the dairy industry more profitable by cutting down the cost on investment in raising the sub fertile bulls.

Key Words: bioassay; bull; fertility prediction; in vitro; zona free hamsters oocyte test