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Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 4(8): 441-448
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Bjorn John Stephen1, Manju Singh1, Shoor Vir Singh1*, Saurabh Gupta1, Kundan Kumar Chaubey1, Sahzad1, Sujata Jayaraman2, Mukta Jain2, Jagdip Singh Sohal2, Shivalingappa Yamanappa Mukartal3, Kuldeep Dhama4

1Animal Health Division, Central Institute for Research on Goats, Mathura, Uttar Pradesh, India; 2AMITY Institute of Microbial Technology, AMITY University Rajasthan, Jaipur, India; 3Department of Veterinary Microbiology, Veterinary College, Hebbal, Bengaluru, India; 4Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.

Abstract | Paneer (fresh cottage cheese) samples collected from local dairy shops in rural (Farah), peri-urban and urban (Mathura and Agra) regions of South Uttar Pradesh were screened by six tests combinations to estimate the rate of ‘bio-contamination’ and ‘bio-typing’ of MAP (paraTB bacilli). Of 55 paneer samples screened, 54.5, 32.7, 54.5, 74.5, 50.9 and 63.6% were positive by microscopy, IS900 PCR, indigenous ELISA kit, Dot-ELISA, Latex Agglutination test and indirect Fluorescent Antibody tests, respectively. Except 2 (3.6%), all the positive samples were detected by minimum two tests. Of the six test combinations used and compared, microscopy (AFB vs iFAT and IS900 PCR were best combinations for the detection of MAP in fresh cottage cheese (paneer) samples. High rate of bio-contamination of paneer sold by the local dairy shops was due to the use of milk supplies contaminated with high bio-load of MAP. Milk supplies were sourced from domestic livestock belonging to local rural areas, where animal health care facilities and knowledge on bio-contamination of MAP were non-existent. Therefore, in order to produce clean milk, free of MAP bacilli it is absolutely essential to control infection in the domestic livestock.

Keywords | Mycobacterium avium subspecies paratuberculosis, Indigenous plate ELISA, Dot- ELISA, Latex agglutination test, Indirect Fluorescent antibody test