Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 4 (6): 294 - 300
Http://dx.doi.org/10.14737/journal.aavs/2016/4.6.294.300
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Kodakkadan Manikkan Dileepkumar1, Swapan Kumar Maiti2*, Kumar Naveen2, Ajantha Ravindran Ninu3, Abhijit Mitra2

1Department of Veterinary Surgery & Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala, India; 2Surgery Division, Indian Veterinary Research Institute, Izatnagar- 243 122, Uttar-Pradesh, India; 3Department of Veterinary Surgery & Radiology, Veterinary College & Research Institute, Tirunelveli, TANUVAS, Tamil Nadu, 627358, India.

Abstract | Mammary neoplasms are the most frequently observed neoplasms in female dogs and constitute an important problem in veterinary medicine. The objective of this study was to investigate the p53 and Cox-2 gene expressions profile in mammary tumours of dogs of different histological types in comparison to healthy mammary tissues using quantitative real time PCR (qPCR) assay. A total of 12 cases (5 benign lesions: adenomas and benign mixed tumour and 7 malignant lesions: carcinomas and malign mixed tumour) were examined. Higher expression levels for p53 and Cox-2 genes in mammary tumour tissues were observed as compared to normal mammary tissues. Expression of both genes was relatively higher in malignant tumour than benign. These results show that p53 and Cox-2 genes may be useful tumour markers for early evaluating malignancy in canine mammary tumours.

Keywords | Dog, mammary tumours, p53, Cox-2, tumour markers