Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 3 (10): 550 - 558
http://dx.doi.org/10.14737/journal.aavs/2015/3.10.550.558
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Khalisa Khadim Khudiar*, Aous Muhammad Ali Aldabaj

Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Baghdad-Iraq.

*Correspondence | Khalisa Khadim Khudiar, University of Baghdad-Iraq; Email: khuder@gmail.com

Abstract
The present study was undertaken to search out the beneficial effect of natural antioxidant (Grape seed oil) against deleterious effect of fluoridation (sodium fluoride in drinking water) on hepatic function of adult male rabbits. Thirty adult male rabbits were randomly divided into three groups (C, T1 and T2) and were treated daily for 60 days as follows: Group C: Rabbits of this group were allowed to ad libitum supply of drinking water (control group), treated groups (T1 and T2) were given drinking water containing 100 ppm sodium fluoride (NaF), in addition to NaF, rabbits in group T2 were received 0.35 ml /Kg B.W of grape seed oil (GSO). Fasting blood samples were collected at different times 0, 30 and 60 days of the experiment by cardiac puncture technique for measuring the following parameters: Serum reduced glutathione (GSH), total protein (TSP) and blood glucose concentrations, as well as, serum aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities were estimated. In addition, sections from liver were taken at the end of the experiment for histopathological study. The results revealed that animals exposed to NaF (T1 group) in drinking water for 60 days showed hepatic dysfunction manifested by significant elevation in the serum AST and ALP activities with a significant decrease in serum protein and significant increase in blood glucose concentrations. A significant reduction in serum GSH concentration were also clarified comparing to T2 group. While oral intubation of grape seed oil concurrently with NaF caused a significant correction of the previous studied parameters related to hepatic functions manifested by significant decrease in serum AST and ALP activities, hypoglycemia with significant elevation in serum GSH, and TSP concentrations. Histological sections of liver in NaF treated (T1) group revealed hepatic injury manifested by mononuclear cell aggregation around the congestive blood vessel and bile duct in the buccal area together with dilatation of the sinusoid.

Keywords | Grape seed oil, Sodium fluoride, Liver enzyme, TSP, GSH, Rats

Editor | Kuldeep Dhama, Indian Veterinary Research Institute, Uttar Pradesh, India.

Received | July 11, 2015; Revised | August 08, 2015; Accepted | August 09, 2015; Published | August 28, 2015

Citation | Khudiar KK, Aldabaj AMA (2015). Effect of grape seed oil on hepatic function in adult male rabbits treated with sodium fluoride (Part-II). Adv. Anim. Vet. Sci. 3(10): 550-558.

ISSN (Online) | 2307-8316; ISSN (Print) | 2309-3331

Copyright © 2015 Khudiar and Aldabaj. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.