Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 3 (4): 253 - 258
http://dx.doi.org/10.14737/journal.aavs/2015/3.4.253.258
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Mukesh Bhakat1*, TK Mohanty2, S Singh1, AK Gupta3, AK Chakravarty3, P Singh1

1Artificial Breeding Research Centre; 2Livestock Research Centre, Livestock Production Management; 3Dairy Cattle Breeding Division, ICAR-National Dairy Research Institute, Karnal – 132001, Haryana, India.

*Correspondence | Mukesh Bhakat, ICAR-National Dairy Research Institute, Karnal, Haryana, India; Email: bhakat.mukesh@gmail.com

Abstract
A total of 156 ejaculates each from 8 Murrah buffalo and 8 Crossbred (Karan Fries) bulls were collected during one year period at Artificial Breeding Research Centre, NDRI, Karnal, India and analysed using least squares analysis to quantify the effect of collector on various semen quality parameters. The overall least squares mean values of Murrah buffalo and Crossbred bulls for ejaculate volume (ml), mass activity, Initial motility (%), sperm concentration (106/ml), total sperm output (106), non-eosinophilic count (%), HOST (%), acrosome integrity (%), head abnormality (%), mid-piece abnormality (%), tail abnormality (%), total abnormality (%), pH and osmolality (mOsmol/kg) were 2.66±0.10 & 4.45±0.10; 2.54±0.70 & 1.79±0.78; 60.64±0.02 & 46.73±0.03; 1016.68±21.25 & 839.25±27.38; 2748.67±122.86 & 3689.55±146.17; 67.20±0.03 & 53.38±0.04; 52.72±0.01 & 40.88±0.03; 70.10±0.02 & 55.73±0.04; 2.30±0.001 & 3.16±0.02; 1.62±0.01 & 2.23±0.007; 5.50±0.002 & 7.53±0.001; 9.47±0.002 & 12.97±0.001; 6.78±0.20 & 6.91±0.19 and 277.78±2.40 & 287.30±2.34, respectively. Collector was found to have significant (p < 0.01) influence on all semen quality traits of Murrah buffalo bulls, but there were no significant differences (p<0.05) between collector 1 and collector 2. In case of Karan Fries bulls, the collector had significant (P<0.05) effect on ejaculate volume (4.84 vs. 4.06 ml) and total sperm output (4032.19 vs. 3346.19 106), but did not have significant (P<0.05) effect on other semen quality parameters. It was concluded that semen collection is highly skilled and scientific job and need efficient collector to achieve collection of optimum quantity and quality of semen, which is the major aim of semen stations.

Keywords | Collector, Motility, Semen quality, Karan Fries and Murrah buffalo bull