Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 1 (2): 53 - 58
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Pawan Kumar1, Nagarajan. N2., Deepak Kumar3, Raj Bahadur Bind4, Ramesh Somvanshi5
1,2,4,5Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India; 3Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India- 243 122
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The present study describes the etiopathological characterization of cutaneous warts (CWs) of cattle and buffaloes. A total of 24 wart samples (cattle-15 and buffaloes-9) were studied. Grossly, most of CWs were of variable sizes, rough or coarse in texture, grayish/blackish/flesh coloured, irregular in shape (dome or button) or resembling cauliflower-like masses and elevated from skin surface by broad base. Fibropapilloma was the most frequent histological type diagnosed. PCR was performed to detect the presence of BPVs and it revealed that CWs of 14 cattle were positive for BPV-2 while 5 samples were found to have mixed infection as they were also positive for BPV-1. Out of 9 CWs samples of buffaloes, 5 were found positive for BPV-1 and 3 for BPV-2. All the samples were negative for the BPV-5 & -10. Quantitative real time PCR revealed that DNA samples of cattle warts had comparatively higher viral load than those of buffaloes. Immunohistochemistry revealed that the PCNA and Ki67 immunopositivity was present in the basal and spinosum layer, respectively, of the fibropapilloma/papilloma. In conclusion, BPV types prevalent in the CWs of Indian cattle and buffaloes population are BPV-1 & -2 and Ki 67 may have association with viral replication as it expressed in spinosum layer where viral replication and assembly occurs.

Key Words: papillomavirus, cutaneous papillomatosis, fibropapilloma, Real time PCR, Immunohistochemistry