Advances in Animal and Veterinary Sciences

Short Communication
Adv. Anim. Vet. Sci. 1 (1): 44 - 46
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Ajay Kumar Rai, Vinod Kumar Chaturvedi*, Thangalazhy Gopakumar Sumithra, Sunita Chougule, Bincy Joseph, Murugan Shanmugam
Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar -243122, Uttar Pradesh, India
*Corresponding author:

In order to overcome the economic difficulties associated with large scale production of enterotoxaemia vaccines, an attempt was made to optimize the in-vitro growth of Clostridium perfringens type D, so as to elevate antigenic mass of the vaccine. Maximum growth density was obtained in the production medium supplemented with 0.2% sucrose and 0.2% vitamin mixture with pH adjustment (7.5-8.0) after 8h of incubation. Growth density in these conditions was about two times more, than the growth at ordinary conditions. Among sugars tested (glucose, sucrose and dextrin), sucrose was the better growth substrate. When pH of media was controlled to 7.5-8.0 after 8h of incubation, growth was approximately one and half times more compared to same medium with uncontrolled pH. Mixture of vitamin B complex and C showed a stimulatory effect when supplemented with sugars. The optimum temperature was found to be 41°C. Sodium chloride had the utmost effect at 0.75% concentration. Tween-80 and sodium acetate were proved as inhibitors.

Key Words: Enterotoxaemia vaccine, C. Perfringens Type D, Sucrose, Sodium chloride, Sodium acetate, Tween-80