Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 2 (8): 457 - 463
View Full HTML
Download PDF

Karthikeyan Jagadeesan, Thooyavan George,Mullai Nila Kamalanathan, Ilayaraja.T
Department of Zoology, Presidency College (Autonomous), Chennai – 600 005, India.

Elytraria acaulis, a traditional plant used to cure worm infection has been evaluated for its anthelmintic and antioxidant activity. Adult parasites of Moniezia expansa collected from the gut of Ovis aries were exposed to 25, 50, 100 mg/ml concentration root extracts. Albendazole concentration of 10 mg/ml was taken as positive control, with negative control of PBS in 1%DMSO. Physical activity, concentration of carbohydrate and protein along with the light microscopic observations were taken as the parameters to assess the anthelmintic potential of Elytraria acaulis. The control group parasites incubated with 1% DMSO (Dimethyl Sulfoxide) survived for more than 58 hours. In vitro treated parasites showed a dose dependent activity on the onset of paralysis, with 100mg/ml concentration. Paralysis was observed after 32.95±0.095 and 48.40±0.141 minutes. With reference to methanol and ethanol extracts respectively, the parasites incubated with methanol extract, the death of the parasites were recorded after 45.05±0.122 min. The concentration of carbohydrate and total protein levels were marginally elevated after treatment. Deformation in the subtegument layer along with the vacuolization of parenchymal tissues was evident. The antioxidant activity of the methanol extract of the plant shows a free radical scavenging activity of 79.38% with 200 μg/ml concentration. Anthelmintic and antioxidant efficacy of the tested plant is because of the presence of various phytoconstituents. Methanolic root extract of Elytraria acaulis seems to be a potent agent for anthelmintic with antioxidant activity. Further studies may throw light on the active compounds present in the crude extracts.