Advances in Animal and Veterinary Sciences

Review Article
Adv. Anim. Vet. Sci. 2 (4): 255 - 260
http://dx.doi.org/10.14737/journal.aavs/2014/2.4.255.260
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Namdeo Rajendra Bulbule, Sunil Sanjay Kapgate, Milind Madhukar Chawak*
Poultry Diagnostic and Research Center, Division of Venkateshwara Hatcheries Private Limited, Loni–Kalbhor, Pune 412 201, India
*Corresponding author: chawakmm@rediffmail.com

ABSTRACT
Gout in poultry is multifactorial in origin and can be associated to many infectious, nutritional, toxic causes or in combination that impair renal functions or cause damage kidney. The infectious agents commonly involved in the pathogenesis of gout are the nephropathogenic infectious bronchitis, and avian nephritis virus. In addition, the chicken astrovirus, found to cause gout in field and experimental cases in commercial broilers in India. The chicks up to the age of 10 days dies abruptly, gross lesions are visceral and articular gout. The pathogenicity study of the chicken astrovirus using kidney sample and virus isolates along with its molecular confirmation by RT–PCR followed by sequencing confirms that chicken astrovirus as a causative agent. Diagnosis of chicken astrovirus and avian nephritis virus from gout affected chickens can be made using degenerate RT–PCR primers targeting the polymerase gene. Reverse transcriptase polymerase chain reaction (RT–PCR) or quantitative real time PCR (qRT–PCR) are useful to differentiate avian nephritis viruses and chicken astrovirus RNA in the original tissue inoculums and also for confirmation of virus isolation. Chicken astrovirus is also important causative agent for gout or nephritis problems in addition to avian nephritis virus and infectious bronchitis virus.

Key Words: CAstV, IBV, ANV, Gout, RT–PCR