Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 2 (4): 242 - 247
http://dx.doi.org/10.14737/journal.aavs/2014/2.4.242.247
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Muhammad Abbas1,5, Muhammad Zubair Shabbir 2, Syed Abdul Khaliq 1,5, Sajjad Ali 3, Muhammad Munir4*
1Quality Control Laboratory, Veterinary Research Institute, Lahore 54000, Pakistan; 2University of Veterinary and Animal Sciences Lahore 54600, Pakistan; 3Disease Investigation Section, Veterinary Research Institute, Lahore 54000, Pakistan; 4Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, 751 89 Uppsala, Sweden; 5Livestock and Dairy Development Department, Punjab, Pakistan
*Corresponding author: drmunir.muhammad@gmail.com

ABSTRACT
Newcastle disease (ND) is a highly contagious disease that results in huge economic losses to the poultry industry worldwide including Pakistan. Despite the disease remained endemic, the genetic nature of the hemagglutinin–neuraminidase (HN) gene of NDV waited investigations in several Asian countries. This study was designed to gain insights into the genetics of HN gene of NDV isolated from Pakistan. Sequence analysis of the HN gene of NDV, isolated from thirteen outbreaks in broiler breeder (n = 3) and broiler chickens (n = 10), was performed. On the basis of clinical symptoms, mean death time (MDT, ~ 47 hours) and intracerebral pathogenicity index (ICPI, 1.5), the isolates were found to be velogenic. The phylogenetic analysis indicated a close relationship studied isolate to the Swedish (Chicken/Sweden/97) and Russian isolates (Sterna/Astr/2755/2001). A parallel comparison of HN gene of Chicken/Pak/2011 with other representative of known genotypes of NDV revealed 15 consistent nucleotide substitutions. Beside these substitutions, important mutations were observed at the crucial sites in the HN protein. This data provide foundation on the evolution of HN gene, possible role of HN gene in disease occurrence and emergence of novel genotypes of NDV.

Key Words: Newcastle disease, Outbreaks, Hemagglutinin, Neuraminidase, Sequence Analysis, Pakistan