Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 2 (4): 199 - 203
http://dx.doi.org/10.14737/journal.aavs/2014/2.4.199.203
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Mohamed Sabry AbdElraheam Elsayed1, Eman Abdeeen1, Mohamed Ali Akiela2, Thaer Farouk3, Rasha Zahran1
¹Department of Bacteriology, Mycology and Immunology faculty of veterinary medicine Sadat city University, Menoufyia governorate, Egypt; 2Department of microbiology faculty of veterinary medicine Alexandria university; 3Administration laboratories in Alexandria port4, Egypt
*Corresponding author: mohammedelsayed529@yahoo.com; mohamed.sabry@vet.usc.edu.eg

ABSTRACT
This study was aimed to detect Salmonella spp. in imported frozen beef and buffalo meat cuts from India, Brazil, Australia and Newzeland. One hundred frozen meat samples were collected from supermarkets located at Alexandria province and from Alexandria port. Salmonella was biochemically identified in 18/100 (18%) samples. Serologically, S. paratyphi A and S. typhi were detected in 10/18 (55.56%) and 8/18 (44.44%) positive samples, respectively. Using conventional PCR, 18/18 (100%) were confirmed to be pathogenic by specific primer to rfbS gene of the pathogenic strains of Salmonella responsible for the most food poisoning while all of these pathogenic isolates 18/18 (100 %) were also confirmed by real time PCR amplifying the invA gene of Salmonella spp.

Key Words: Imported meat, Salmonella spp., Conventional PCR, Real time PCR