Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 2 (4): 192 - 198
http://dx.doi.org/10.14737/journal.aavs/2014/2.4.192.198
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Harshad C. Chauhan1, Hemendra Singh Kher1, Kaushal Kishor Rajak2, Arnab Sen2, Abidali I Dadawala1, Bharat Singh Chandel1
1Department of Microbiology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University Sardarkrushinagar– 385 506– Banaskantha, Gujarat, India; 2Division of Virology, Indian Veterinary Research Institute, Mukteswar–Nainital, India
*Corresponding author: hcchauhan1972@gmail.com

ABSTRACT
The present study reports the diagnosis of PPRV infection in sheep and goats using serological, molecular and isolation methods. Out of 618 sera screened for presence of PPRV antibodies by c–ELISA in sheep and goats, 324 (52.42 %) were found positive. Among 268 ante mortem samples, 63 (23.50 %) were found to be positive, whereas in post mortem samples 21 (24.70 %) yielded positive results. A high percentage of positive samples were found in sheep (21.68 %) compared to goats (27.55 %). Among the tissue samples tests, lymphnodes yielded highest positivity (71.42 %), followed by spleen (50.00 %), lung (40.00 %), intestine, liver, heart (each 28.57 %) and trachea (20.00 %). Based on the genetic detection of the N gene, 12 samples comprising four nasal swabs, one faecal material, two spleen, two heart, two lymphnodes and one liver were found to be positive for PPRV. The PPRV was successfully isolated from two nasal swabs of goats, and spleen and heart samples of sheep at 6th passage in Vero cells. Relative quantification of PPRV in various tissues from natural outbreaks revealed highest viral load in lymphnode and lung samples.

Key Words: PPRV, RT–PCR, S–ELISA, Real time PCR, Isolation, Gujarat