Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 2 (3): 177 - 182
http://dx.doi.org/10.14737/journal.aavs/2014/2.3.177.182
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Pramod Kumar Nanda2, Priyabrat Swain*1, Sukanta Kumar Nayak1, Sudhansu Sekhar Mishra1, Pallipuram Jayasankar1, Sanjeeb Kumar Sahoo3
1Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar–751 002, India; 2Indian Veterinary Research Institute, Eastern Regional Station, Belgachia-700 037, Kolkata, India; 3Institute of Life Sciences, Bhubaneswar –751 023, India
*Corresponding author: pswainy2k@yahoo.co.in

ABSTRACT
Cell culture has many applications in biological research. Now days, the focus has been to replace routinely practiced two–dimensional (2–D) cell culture with three–dimensional (3–D) cell culture systems due to their efficacy in increasing cell attachment, proliferation and differentiation. In this study, formulated poly lactic acid (PLA) scaffold blended with different natural polymers such as gelatin (PLA–G), alginate (PLA–A), dextran (PLA–D) and pectin (PLA–P), by multiple emulsion solvent evaporation method, were evaluated to find their effect on the attachment, growth and proliferation of fibroblast–like cells from heart of Indian Major Carp, Cirrhinus mrigala. Amongst the formulated scaffolds, PLA–A demonstrated significantly better growth of fibroblast–like cells as compared to control and other formulations. The effect of different seeding density (0.4, 0.6, 0.8, 1.2 and 1.6 × 10 5 cells ml–1 media) on cell proliferation and growth was also studied using PLA–A. PLA–A demonstrated more than 1.5 times higher cell proliferation and growth as compared to control (2–D) cell culture after 9th day post seeding, even with less cell seeding density (1.6 × 105 cells ml–1 media). However, with a seeding density of 0.8 × 105 cells ml –1 media, this scaffold formulation (PLA–A) supported best proliferation and growth of fibroblast cells. In short, PLA–A based hydrophilic scaffold formulation revealed its potential use in culture of anchorage dependent fibroblast–like cells from heart of Indian major carp, C. mrigala.

Key Words: Cirrhinus mrigala, Fibroblast–like cells, Growth characteristics, In vitro cell culture, Polymeric scaffolds