Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 10(1): 78-84
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Hernando Daniel Acevedo1*, Sirley Adriana Ortiz2,3, Polyana Galvão Coelho2, Iang Schroniltgen Rondón-Barragán1, Marlene Isabel Vargas2

1Faculty of Veterinary Medicine and Zootechnics, University of Tolima (UT), Ibagué –Tolima, Colombia (Acevedo, Rondón-Barragán); 2Department of Veterinary, Federal University of Viçosa (UFV), Viçosa Minas Gerais, Brazil (Vargas, Ortiz, Coelho); 3Program of Veterinary Medicine and Zootechnics, University of Santander (UDES), Campus Valledupar, Colombia (Ortiz).

Abstract | There are a significant number of patients with canine mammary tumors (CMTs) whose outcome and prognosis are not predicting with the clinical indicators and current histopathological grading (I, II, III), where grade III mammary carcinomas have a 21-fold greater risk of death than grade I and II tumors. Therefore, it is main to use complementary diagnostic methods to improve histological classification in these cases. The presence of type I (Coll-I) and III (Coll-III) collagen in the tumor stroma is related to metastasis from human breast cancer. From paraffin-embedded samples (FFPE) of the stroma of canine mammary gland carcinomas (CMGCs), we describe the characterization of type I and III collagen fibers in 10 samples of each histological grade (n=30), using Picrosirius red (PSR) staining under polarized light to evaluate its relationship with histological grading. The Coll-I fibers showed different lengths, disposition, and thickness in all tumor grades with a random distribution. In the three histological grades, the Coll-III fibers showed a lower expression than the Coll-I fibers, showing the highest expression of Coll-III in grade I, decreasing its gradual percentage in grades II and III (P < 0.0001). Our results indicate that the decrease of Coll-III in the tumor stroma is inversely proportional to the increase in the grading of CMGCs. Therefore, we suggest that PSR can be a complementary method that helps to support the grading of CMGCs in a diagnostic laboratory workflow.

Keywords | Oncology, Mammary neoplasm, Canine, Picrosirius.