Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(10): 1601-1607
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Sergey V. Shabunin, Galina A. Vostroilova, Liliya V. Cheskidova, Dmitriy I. Shabanov, Anastasiya A. Korchagina*

All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy, 394087, 114B, Lomonosova Str, Voronezh, Russia.

Abstract | Complex antimicrobial medications containing penicillin group antibiotics in combination with glucocorticoids are often used intramammary for the treatment of mastitis in lactating cows. However, there is insufficient data on the presence of negative and positive effects of their interaction. In this regard, we set the goal of identifying the potential mutagenic properties of the complex medication containing amoxicillin, cloxacillin and prednisolone as active substances. For this purpose, the samples of metaphase chromosomes were made from the bone marrow cells of white mice. The number of cells with chromosomal aberrations was counted. The micronucleus test was carried out in polychromatophilic erythrocytes of peripheral blood obtained from the tail vein of mice. As a result, it was found that subcutaneous administration of the complex medication to white mice for 7 days at a dose of 80 mg/kg of body weight and a single administration at a dose of 560 mg/kg of body weight did not have a mutagenic effect. This is confirmed by the absence of a significant increase in the proportion of bone marrow cells with chromosomal aberrations and the number of polychromatophilic erythrocytes with micronuclei relative to the values of the control group.

Keywords | Complex medication, Genotoxicity, Mice, Penicillins, Prednisolone