Agarose gel electrophoresis for PCR products amplification of the OLR1 gene. Lane M: 100 bp DNA ladder, lanes 1-12 indicate samples of ewes with single birth, lanes 13-25 indicate samples of ewes with twin birth. Electrophoresis conditions: 1.5% agarose concentration, Electrophoresis time 25 minutes. The buffer used TBE pH 8.3. 

SSCP non-denaturing polyacrylamide gel electrophoresis of ovine OLR1 gene (exon 3) PCR fragments, which appeared two PCR-SSCP banding patterns, CC and AC in Awassi ewes.