Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(9): 1408-1415
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Ismail A. Radwan1, Salama A.S. Shany2, Sara S.E. Amin1, Ahmed H. Abed1*

1Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt; 2Poultry Diseases Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.

Abstract | Pseudomonas aeruginosa is an opportunistic environmental pathogen causing serious problems in poultry farms. Our study investigated the prevalence of P. aeruginosa in 80 examined kidney samples of broiler chicken where 6 P. aeruginosa isolates (7.5%) were isolated. The extended spectrum β-lactamases (ESβLs) production was detected phenotypically in P. aeruginosa isolates using modified CLSI ESβLs confirmatory test and 66.7% of isolates were ESβLs producers. PCR was conducted on all isolates for detection for blaTEM and exoS genes that were found in 66.7 and 100% of isolates, respectively. Antibacterial activity of carvacrol oil was tested against all P. aeruginosa isolates at concentrations of 800, 400, 200, 100 and 50 µl/ml. Concentrations of 800, 400 and 200µl/ml showed complete growth inhibition while 100 and 50 µl/ml concentrations inhibited the growth of 66.7% and 33.3% of isolates, respectively. Real time-PCR (RT-PCR) was conducted on the non-inhibited P. aeruginosa isolates at 100 µl/ml concentration after treatment with carvacrol oil for detection of the possible effect on blaTEM and exoS genes expression and the results indicated mild reduction of both genes expression after treatment (0.6-0.8 folds). It was concluded that carvacrol oil could be used as alternatives for synthetic antimicrobial drugs.

Keywords | P. aeruginosa, ESβL, blaTEM gene, exoS gene, Carvacrol, Broiler chickens, Kidney lesions