Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(9): 1383-1389
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Khaled Mohamed El-Dakhly1*, Magdy M. Tawfik1, Amany Samir Aboshinaf2, Lilian N. Mahrous1, Waleed M. Arafa1

1Department of Parasitology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt; 2Provincial Laboratory of Animal Health Research Institute, Agriculture Research Center, Dokki, Giza, Egypt.

Abstract | Anaplasmosis and ehrlichiosis are important tick-borne diseases in dogs. The current study aimed to evaluate the occurrence of anaplasmosis and ehrlichiosis in owned dogs in Alexandria, northern Egypt. Accordingly, a total of 70 dogs, admitted to private clinics for veterinary care, were surveyed and blood specimens were taken for both Giemsa-stained microscopic examination and molecular detection using conventional PCR. The present study revealed that 8.57% (6/70) dogs were infected with Anaplasma/Ehrlichia spp. Based on the examination of Giemsa-stained blood smears, Anaplasma platys was found in blood platelets of 4 (5.71%) dogs and Ehrlichia canis was revealed inside macrophages of 2 (2.86%) dogs. No mixed infections were recovered. All infected dogs had an infestation with Rhipicephalus sanguineus ticks. Variable clinical signs were detected in the surveyed dogs, often loss of appetite, loss of body weight and lethargy. Among risk factors, it has been found that dogs aged less than 2 years were the highest infected as well as male dogs were more susceptible to the infection. cPCR-based molecular investigation targeting the 16sRNA gene revealed that 20.0% (14/70) of dogs were positive for Anaplasma/Ehrlichia species with a specific amplicon size of 345 bp. Expectedly, the use of PCR-based molecular tool was more accurate than the conventional microscopic examination. Further literature based on both traditional and molecular tools targeting another hemoparasites infecting dogs together with an accurate identification of the associated tick vector are urgently needed to achieve a successful control of those pathogens.

Keywords | Anaplasma platys, Ehrlichia canis, Dogs, Microscopy, PCR, Egypt