A.Imsya1, Muhakka1, Yuanita Windusari2

Abstract | This study was aimed at obtaining the most appropriate balance of soluble carbohydrate (SCH) and protein supplementation to optimize the in vitro utilization of gradedly ammonized total mixed fiber (TMF) originating from agricultural wastes and swamp grasses in rations of Pampangan swamp buffaloes. In this study, optimization was done on TMF ammonization by using 12% graded level of urea (2, 4, and 6%). A completely randomized design with 3 treatments and 6 replicates was used. Treatments consisted of 3 levels of SCH and protein (P) balances, namely no supplementation (A0), 600 g SCH and 300 g P (A1), and 900 g SCH and 300 g P (A2), Measurements were taken on digestibility of nutrients including dry matter, organic matter, crude protein, fiber, NDF, and ADF and characteristics of rumen condition including RUN concentration, total VFA concentration, partial VFA concentration, rumen pH, and population of rumen microbes. Results showed that supplementation of SCH and protein balances gave significant effects (P< 0.05) on digestibility of nutrients, fiber fractions, and characteristics of rumen condition in swamp buffaloes fed basal ration of gradedly ammonized TMF. Increased SCH and protein supplementation in ration improved digestibility (dry matter, organic matter, NDF, ADF cellluoce) and concentration of RAN and total VFA It was concluded that SCH and protein balance of 3:1( 900g SCH and 300g protein) was the best SCH and protein supplementation in rations of swamp buffaloes fed basal ration of gradedly ammonized TMF

Keywords | Gradedly Ammonization, Soluble Carbohidrate (SCH), Suplemmentation, Total Mixed Fiber (TMF), Swamp Buffalo.