Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(8): 1135-1142
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Manar A. Khader1, Magdy F. El-Kady2, Iman B. Shaheed3*

1Department of Pathology, Faculty of Veterinary Medicine, Al Azhar, University, Gaza, Palestine; 2Department of Poultry Diseases, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt; 3Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.

Abstract | The control of Newcastle disease virus (NDV) depends on vaccination to protect chickens against clinical signs and mortalities. Live vaccines are usually used due to it slow cost and easy application. This study was performed to evaluate post-vaccinal reactions of LaSota and VG/GA (AVINEW®) vaccines in broiler chickens. One hundred twenty day- old commercial broiler chicks were divided into three groups (40 each). Group 1 maintained as non-vaccinated group (negative control), Group 2 was vaccinated with LaSota vaccine while Group 3 was vaccinated with AVINEW® vaccine by ocular rout at day 10 of age. All chicks were vaccinated with infectious bursal disease vaccine at 12th day. Blood samples were collected weekly from each group. Three chicks were sacrificed from each group at 2-, 5-, 10- and 15-days post vaccination (dpv). Tissue samples were collected from trachea, kidneys, proventriculus, small intestine, spleen and Harderian gland for histopathological examination. Neither clinical signs nor mortalities were recorded in vaccinated groups. Petechial hemorrhage in Harderian gland was detected in both vaccinated groups. No significant difference in antibody titers was observed between both vaccinated groups. Histologically, edema, inflammatory cells infiltration, and focal deciliation were detected in trachea of both vaccinated groups. Avinew® vaccine produced more potent post-vaccinal reaction than LaSota in kidneys and small intestine. Meanwhile reactions on trachea, proventriculus, spleen and Harderian gland were almost identical.

Keywords | Histopathology, LaSota, NDV, Spleen, Trachea