Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(7): 1078-1086
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Hend Mohamed Hussein1*, Nehal A Radwan2, Noha Abdelfattah Elleboudy1*, Reham Fathy Tash3, Youssef Shoukry3, Ahmed H Abdelazeem4,5, Shimaa Abdelraouf Elgohary2

1Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo 11591, Egypt; 2Department of Pathology, Faculty of Medicine, Ain Shams University, Cairo 11591, Egypt; 3Department of Anatomy, Faculty of Medicine, Ain Shams University, Cairo 11591, Egypt; 4Department of Medicinal Chemistry, Faculty of pharmacy, Beni-Suef University, Beni-Suef 62514, Egypt; 5Department of Pharmaceutical Sciences, College of Pharmacy, Riyadh Elm University, Riyadh 11681, Saudi Arabia.

Abstract | Osteopontin is a granulomatogenic chemokine considered to be an inflammatory progenitor suggested to participate in hepatopathology in schistosomiasis. The aim of the current study is to evaluate Osteopontin immunohistochemical expression in chronic murine Schistosoma mansoni infection through week 7 till week 23 in both liver and colon histopathology samples, correlating them to serum glutamic-oxaloacetic transaminase and glutamate pyruvate transaminase levels. The results revealed significant association between levels of SGOT and SGPT and percentage of liver inflammation (P =0 .037 and 0.002 respectively), also smaller and bigger sized granulomas were significantly linked to low level of SGPT (P = 0.005). A parallel histopathologic change over time was detected in both liver and colon specimens. The infected groups show acute granuloma formation associated with liver fibrosis from week 7 through week 23. Changes in the expression of Osteopontin was observed at week 7. Upregulated expression was strongly seen from week 11 to week 13. At week 13 to week 17 the fibrotic granulomas reveal many positive cells, from week 21 to week 23 the expression was weak and almost was returned to near normal levels. Intensity of the expression was positively significantly correlated with the degree of inflammation, granuloma size and the degree of fibrosis (P < 0.01). This study highlights the use of OPN as a potential diagnostic marker of liver injury and the probability of using an anti OPN in early chronicity stage to prevent schistosomal subsequent pathological changes.

Keywords | Schistosoma mansoni, Osteopontin, Granuloma, Liver fibrosis, Colon.