Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(7): 994-1003
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Nedaa Ali Eldesouky1, Gehad E. Elshopakey1, Mohamed S. Yusuf2, Fatma M. Abdelhamid1, Engy. F. Risha1*

1Department of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt; 2Nutrition and Clinical nutrition Department, Faculty of Veterinary Medicine, Suez Canal University, 41522, Ismailia, Egypt.

Abstract | This study conducted to assess the effect of vitamin D deficiency on progression of CCl4 mediated hepatic oxidative stress and inflammation in male albino rat. Forty male albino rats, weighing 120-170g, were randomly divided into four equal groups (n= 10/group):control group, vitamin D deficiency group, CCl4 treated group (1ml CCl4: 1 ml olive oil), at dose 0.2 ml /kg BW once daily and the vitamin D deficiency+CCl4 one group. After six weeks of treatment, biochemical parameters, hepatic oxidative and, antioxidant biomarker, calcium, phosphorus, and Vit D were determined. Our result showed that CCl4+Vit D deficiency and CCl4 treated groups showed significant elevation in the serum ALT, AST and ALP with significant decrease in the total protein and globulin compared with the control group. A significant elevation in total, indirect bilirubin and direct bilirubin level was observed in the CCl4+Vit D deficiency group compared to CCl4 group. Vitamin D level was significantly declined in all experimental groups compared to control group. Moreover, calcium level was decrease in Vit D deficiency group and CCl4+Vit D deficiency group compared to the control. The hepatic antioxidant biomarkers (GSH and SOD) were significantly reduced in CCl4 and CCl4+Vit D deficiency groups, while hepatic MDA level was significantly increased in the same groups unlike the control group. Overall, our findings revealed that the co-existence of vitamin D deficiency might aggravate hepatic inflammation and necrosis; this was confirmed by our biochemical, oxidative stress/antioxidant and histopathological results.

Keywords | Hepatic toxicity, CCl4, Vitamin D deficiency, Oxidative stress, Rats