Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(7): 964-970
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Nguyen Van Vui*, Nguyen Thi Kim Quyen, Nguyen Thuy Linh, Nguyen Thi Mong Nhi

Tra Vinh University, No. 126 Nguyen Thien Thanh Street, Ward 5, Tra Vinh City, Tra Vinh Province, Vietnam.

Abstract | Canine sperm are susceptible to oxidation due to the unbalance between the high level of ROS and inadequate antioxidant protection during preservation. To improve the quality of chilled canine sperm, the supplementation of eugenol and vitamin E in the Tris-citric-fructose-mineral salts egg-yolk extender was conducted to evaluate the effects them on canine sperm quality during storage. Twelve ejaculates from three American Bully dogs were used. The sperm motility parameters were analysed by using computer-assisted sperm analysis (CASA), whereas the plasma membrane integrity, acrosome membrane integrity, and mitochondrial membrane potential parameters were identified by using a confocal laser scanning microscope. Thiobarbituric acid (TBA) assay was used to determine the level of sperm lipid peroxidation. The results showed that the sperm quality parameters in all the semen extenders decreased gradually during the whole storage time of 12 days. Besides, there was no significant difference in the values of chilled canine sperm motility parameters among all the treatments (P>0.05). Moreover, the percentage of the intact plasma membrane, intact acrosome membrane, and high mitochondrial membrane potential parameters in the eugenol treatment were better than those in the rest treatments (P<0.05). In conclusion, the eugenol is greater than the vitamin E to protect canine sperm during cooling preservation.

Keywords | Eugenol, Vitamin E, Antioxidants, Semen extenders, Canine sperm