Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(3): 453-461
Http://dx.doi.org/10.17582/journal.aavs/2021/9.3.453.461
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Alsagher O. Ali1, Waleed Younis2, Hassan Y.A.H. Mahmoud1*

1Division of Infectious Diseases, Animal Medicine department, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt; 2Departments of Microbiology, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt.

Abstract | S. aureus is one of the most common organisms associated with infections among dairy animals with frequent and rapid development of antibiotic resistance. The presence of genes encoding aminoglycoside resistance tetracycline resistance, and beta-lactam resistance were detected by whole genome sequencing. Seventy-nine S. aureus clinical isolates were isolated from milk samples of dairy cattle and she-camels in Southern Egypt. The sequences of resistant genes extracted from the whole genome sequences of the bacterial chromosome that was done through shotgun sequencing, through phylogenetic analysis, all resistant genes sequences were arranged into two main clusters. The first clusters contain mecA genes, aminoglycosides, fusB gene as well as blaz gene except A11, and two reference genes (AJ302698 and AP003139). The second cluster contains mainly the tet(k) and tet(38) genes which are more similar to each other, in addition to mecA reference gene (AB512767) and two blaz reference genes (AJ302698 & AP003139). The phylogenic tree revealed that all resistant genes are more or less stable genes such as mecA, aminoglycosides, tet(k), tet(38), and fusB except blaz gene showing more variations. Molecular detection continues to be increased to establish antimicrobial resistance trends for S. aureus found in subclinical mastitis of dairy animals.

Keywords | Antibiotic resistance, Camel, Cattle, S. aureus, Egypt