Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 8(s2): 24-33
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Ayman A. Khattab1, Azza M. Tawfek2, Khaled Abo-EL-Sooud3*, Kawkab A. Ahmed4, Abd El Nasser El-Gendy5, A.R. Ahmed2

1Egypt Center for Research and Regenerative Medicine, Military Forces, Cairo, Egypt; 2Department of Clinical Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; 3Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza,12211, Egypt; 4Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; 5Medicinal and Aromatic plants Research department, National Research Centre, Dokki, Giza, Egypt

Abstract | Hepatoprotective efficacy of cardamom (Elettaria cardamomum) seeds crude essential oil was evaluated in experimentally paracetamol (PCM) hepatotoxic rats. Sixty-five male Sprague Dawley rats divided into six groups. First group was kept as control negative, while the 2nd group was given PCM (500 mg/kg bw). The 3rd and 4th groups were orally administered silymarin + PCM (500mg/kg bw + 500 mg/kg bw), and cardamom oil + PCM (100 mg/kg bw + 500 mg/kg bw), respectively. The 5th and 6th groups were treated with silymarin (500 mg/kg bw) and cardamom oil (100 mg/kg bw), respectively in normal rats. All the previous dosages lasted for two weeks. Blood samples collected weekly from all tested groups. Assessment of hemogram, some serum biochemical parameters and histopathology of liver and kidney were carried out. Chemical compositions of cardamom oil was established by gas chromatography-mass spectrometry (GC-MS), revealed that the oil was rich in monoterpenoids, while the monoterpene hydrocarbon, was existing with comparatively small percent. Results of PCM-intoxicated rats revealed significant decrease in total protein and albumin with significant hyperglycemia and elevation in serum transaminases, urea and creatinine which were significantly ameliorated in groups receiving cardamom oil or silymarin. Administration of cardamom essential oil signi´Čücantly ameliorates the hepato-renal profiles and elevates total antioxidant capacity. Moreover, the oil protects the liver and kidneys from histopathological alterations in PCM-intoxicated rats. Neither cardamom oil nor silymarin induce any alterations in serum biochemistry and total antioxidant activity in normal rats. The present results proved the ameliorative effect of cardamom essential oil against hepato-renal changes in PCM-intoxicated rats reasonably due to its potent antioxidant effect.

Keywords: Cardamom Oil, Paracetamol, Gas Chromatography-Mass Spectrometry, Antioxidant, Hepato-renal, Rats