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Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 1 (4S): 45 - 50. Special Issue-4 (Progress in Research on Viruses and Viral Diseases)
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Arkendu Halder, Siddhartha Narayan Joardar*, Panchanan Parui1, Dhriti Banerjee1, Vikas Kumar1, Indranil Samanta, Chandan Lodh2
Department of Veterinary Microbiology; West Bengal University of Animal and Fishery Sciences; Post Office –Belgachia, Kolkata–700037; 1Diptera Section, Zoological Survey of India, Post Office –New Alipore, Kolkata–700059, West Bengal, India; 2Department of Veterinary Medicine, Ethics and Jurisprudence, West Bengal University of Animal and Fishery Sciences; Post Office –Belgachia, Kolkata–700037.
*Corresponding author: joardar69@gmail.com

ABSTRACT
With the aim of identifying the potent vector(s) of bluetongue virus (BTV) that is circulating in eastern Indian state, West Bengal, attempts were made to detect and isolate BTV from Culicoides midges, collected from localities having good number of sero–positive animals. Prevalence of various species of Culicoides in the state along with the dynamics of Culicoides population, their habitat and weather parameters were also studied to reveal their possible relation. Culicoides midges were collected from three different agro–climatic zones of West Bengal in different post monsoon months. Meteorological data of collection–months of that area were obtained and incorporated to investigate the environmental conditions at the collection time. Habitat and probable breeding areas of Culicoides sp. were also noted. For isolation of BTV, non–engorged midges were pooled to prepare sample and virus was isolated using embryonated chicken eggs, followed by isolation on cell culture (BHK–21). Confirmation of BTV was made by RNA–PAGE and RT–PCR. The present study reveals the vector competence of Culicoides schultzei that harbored BTV–16. Typical association between the dynamics of Culicoides population, their habitat and weather parameters were observed in the present study. It is concluded that West Bengal state possesses potent vector of bluetongue virus that warrants serious attention in near future.

Key Words: Bluetongue virus, BTV–16, Culicoides sp., Midges, Vector