Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 8(11): 1129-1141
Http://dx.doi.org/10.17582/journal.aavs/2020/8.11.1129.1141
View Full HTML
Download PDF

Nancy B. Mohamed1,2, Amira H. Mohamed2, Nashwa A. Abu-Aita2, Soad M. Nasr3*, Somia A. Nassar3,4, Kawkab A. Ahmed5

1Directorate of Veterinary Medicine, 3 Teraet El-Zomor St., Nasr El Din, El-Haram, Giza, Egypt; 2Department of Clinical Pathology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt; 3Department of Parasitology & Animal Diseases, National Research Centre, 33 Bohouth St., Dokki, Giza 12622, Egypt; 4Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia; 5Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.

Abstract | An experiment was carried out to evaluate Moringa oleifera leaves ethanol extract (MOLEE) as a prophylactic treatment of lead acetate-induced toxicity in rabbits. Serum biochemical parameters, oxidant/antioxidant markers, and histopathology of liver and kidney were determined. The effect of long-term MOLEE treatment was also evaluated. Forty-eight male New Zealand white rabbits were randomly divided into four equal groups: normal control group; MOLEE-treated group, administered MOLEE (orally, 400 mg kg-1day-1) for successive 12 weeks; lead-intoxicated group, administered lead acetate (orally, 40 mg kg-1day-1) for successive 8 weeks; and MOLEE-lead-intoxicated group, administered MOLEE (orally, 400 mg kg-1day-1) for successive 12 weeks simultaneously with lead acetate (orally, 40 mg kg-1day-1) for successive 8 weeks. The results revealed that MOLEE treatment significantly (P<0.05) increased body gain and impacted positively on lipid profile, glucose, liver, and renal functions. On the other hand, administration of lead acetate caused marked (P<0.05) drop in body weight gain and elevated the activities of AST, ALT, and ALP and the levels of urea and creatinine, lipid profile, and lipid peroxidation as well as histopathological changes in the liver and kidneys. After 4 weeks of treatment, MOLEE-lead-intoxicated rabbits showed slight cytoplasmic vacuolization of hepatocytes and slight congestion of glomerular tuft, while vacuolation of epithelial lining renal tubules was noticed in kidneys of the lead-intoxicated group. In conclusion, prophylactic use of MOLEE has the ability to mitigate the alterations of serum biochemical parameters and histopathological architecture of liver and kidneys caused by lead acetate after 4 weeks from the start of treatment. Results of the present experiment revealed that Moringa oleifera has a curing effect on lead toxicity; nevertheless, further studies are required to determine the safety of prolonged use of Moringa oleifera.

Keywords | Lead acetate, Moringa oleifera, Serum biochemistry, Histopathology, Rabbits.