Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 8(8): 868-872
Http://dx.doi.org/10.17582/journal.aavs/2020/8.8.868.872
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Widjiati1*, Zakiyatul Faizah2, Ninik Darsini2, Viski Fitri Hendrawan3, Epy Muhammad Luqman4, Sutiman B. Sumitro5

1Post Graduate School of Universitas Airlangga Surabaya, Indonesia; 2Departement of Biomedical Science Faculty of Medicine Universitas Airlangga Surabaya, Indonesia; 3Departement of Reproduction Faculty of Veterinary Medicine Universitas Brawijaya Malang, Indonesia; 4Departement of Veterinary Anatomy Faculty of Veterinary Medicine Universitas Airlangga Surabaya, Indonesia; 5Departement of Biology Faculty of Science Universitas Brawijaya, Malang, Campus B Universitas Airlangga, Jalan Airlangga 4-6 Surabaya.

Abstract | The weakness of in vitro fertilization (IVF) was not able to observe whether in vitro fertilized oocytes were all mature. Intra Citoplasmic Sperm Injection (ICSI) was able to solve IVF failure as spermatozoa was directly injected to oocyte. The research was divided into 2 groups, T1 group: oocyte was fertilized conventionally (maturated oocyte was supplamanted with spermatozoa) and T2 group: fertilization using ICSI method. Next, oocyte was mixed with spermatozoa. After ICSI was done, it was put into incubator CO2 5 %, with the temperature of 38.5oC. Then, fertilization rate and embryo growth at cleavage stage were observed. The data presented as means Standard Error of the Mean (S.E.M) and were compared using the Student’s t-test. Research result showed that fertilization rate of ICSI group was higher significant than conventional IVF group (p<0.05). Also, cleavage stage (2 cells, 4 cells, 8 cells) of ICSI group was higher significant than conventional IVF group (p<0.05). The research concluded that fertilization rate and embryo cleavage of IVF using ICSI technique were higher than conventional IVF.

Keywords | Sperm injection, Oocytes, Cell block, Fertilization rate, Embryo growth