Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 8(s1): 47-54
Http://dx.doi.org/10.17582/journal.aavs/2020/8.s1.47.54
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Mohamed Fouad Mansour1, Ahmed Hamed Arisha2*, Mohamed Al-Gamal1, Rasha Yahia1, Ahmed A. Elsayed3, Saydat Saad1, Khlood M. El-Bohi4

1Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, 44519 Zagazig, Egypt; 2Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, 44519 Zagazig, Egypt; 3Photochemistry Department, Industrial Chemical Division, National Research Center, Dokki, Giza, 12622, Egypt; 4Department of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Zagazig University, 44519 Zagazig, Egypt.

Mohamed Fouad Mansour and Ahmed Hamed Arisha contributed equally.

Abstract | Testicular toxicity is a significant cause of male infertility. It may occur due to hormonal, nutritional, behavioral, and environmental imbalance. Moringa oleifera, on the other hand, is known to have antioxidant, anti-inflammatory, anti-tumor, anti-hypertensive, anti-diabetic properties. This study assessed the ameliorative effect of Moringa oleifera leaves extract (M/MOLE) on melamine-induced testicular toxicity in rats. Fifty Sprague Dawley (8weeks old) male rats were randomly assigned to group 1 (control), group 2 ( Moringa oleifera leaves extract only), group 3 (administered melamine only), and group 4 (both melamine and M/MOLE). Melamine significantly decreased (P < 0.05) semen quality, testicular weight, gonadal-somatic index, follicle-stimulating hormone (FSH), free testosterone, total testosterone, and an expression of steroidogenic enzyme genes (CYP11A1 and HSD17B3). A concurrent administration of M/MOLE reversed the clinical impact of Melamine treatment (P < 0.05). Histopathological examination revealed an improvement in testicular tissue injury in concurrent M/MOLE treatment groups than the melamine group. In conclusion, MOLE was effective in improving the toxic effects of melamine on testicular tissue in rats.

Keywords | Moringa oleifera leaves, Testicular toxicity, Steroidogenesis, Semen picture