Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 8(s1): 11-17
Http://dx.doi.org/10.17582/journal.aavs/2020/8.s1.11.17
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Sally H. Abou-Khadra1*, Ibrahim M. El-Shorbagy2, Mona M. El-Azzouny1

1Microbiology Unit, Animal Health Research Institute(AHRI), Zagazig branch, Agriculture Research Center (ARC), Egypt; 2Food Hygiene Unit, Animal Health Research Institute(AHRI), Dokii, Giza, Agriculture Research Center (ARC), Egypt.

Abstract | Staphylococcus aureus (S. aureus) produces various types of toxins, that cause food poisoning. This study was designed for a rapid investigation of enterotoxigenic S. aureus from raw milk. One hundred raw milk samples were collected from different farms in Sharkia governorate and examined for genome corresponding to enterotoxin genes (Sea, Seb, Sec, Sed, and See) of S. aureus using multiplex polymerase chain reaction (M-PCR). A reverse passive latex agglutination assay (RPLA) was also used for screening the enterotoxin production. The presence of S. aureus was detected in 20% of the examined samples. The antimicrobial susceptibility patterns showed sensitivity to ciprofloxacin (100%), followed by vancomycin (96%). Maximum resistance was reported for oxacillin (100%), ampicillin (95%) and amoxicillin (90%). Staphylococcal enterotoxin D gene (Sed) was detected in all isolates, while Seb and Sec genes were not detected. Though the results of PCR and RPLA had congruence, a few discrepancies were also reported for some of the isolates. The study concludes the detection of enterotoxigenic S. aureus (Sed and Sea gene) in raw milk samples, and therefore, ascertain necessary interventions to avoid food poisoning.

Keywords | Staphylococcal enterotoxins, Multiplex polymerase chain reaction, Reversed passive latex agglutination, Raw milk