Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 8(5): 531-535
Http://dx.doi.org/10.17582/journal.aavs/2020/8.5.531.535
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Volkan Yılmaz*1, Nüvİt Coşkun1, Mushap Kuru2, Semra Kaya2, Fatİh Büyük3, Özgür Çelebi3

1Department of Virology, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey; 2Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey; 3Department of Microbiyology,, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey.

Abstract | Bovine herpes virus type 1/4 (BoHV-1/4) shows affinity especially to the respiratory and genital system and can cause economic losses by adversely affecting animal health. The purpose of this study was to identify the presence of BoHV-1 and BoHV-4 in leukocyte samples using molecular methods. Therefore, leukocyte samples were collected from 100 cattle with endometritis, not immunized against these infections, in small-scale family farms in the province of Kars, Turkey. The presence of BoHV-1 and BoHV-4 nucleic acids was investigated with polymerase chain reaction (PCR) specific for the Glycoprotein C (gC) and Glycoprotein B (gB) gene, respectively. BoHV-1 nucleic acid could not be detected in any of the leukocyte samples. However, BoHV-4 nucleic acid was detected in 42 cattle with endometritis. Data obtained from this study and identification of the BoHV-4 specific antigen in leukocyte samples in the province of Kars proves that the BoHV-4 infection is present in the region and plays an important role in cases of endometritis observed in cattle.

Keywords | BoHV-1, BoHV-4, Endometritis, Kars, PCR