Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 8(4): 422-427
Http://dx.doi.org/10.17582/journal.aavs/2020/8.4.422.427
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Djalal Rosyidi1, Dahliatul Qosimah2*, Indah Amalia Amri2, Dodik Prasetyo3, Fajar Shodiq Permata4, Agri Kaltaria Anisa5, Lucky Retno Putri2, Nanda Ayu Cindyasputri2, Wulandari2, Yohana Leuricha2, Lilik Eka Radiati1

1Laboratory of Animal Product Technology, Faculty of Animal Husbandry, Universitas Brawijaya, Indonesia; 2Laboratory of Microbiology Dan Immunology, Faculty of Veterinary Medicine, Universitas Brawijaya, Universitas Brawijaya, Indonesia; 3Clinical of Animal, Faculty of Veterinary Medicine, Universitas Brawijaya, Universitas Brawijaya, Indonesia; 4Laboratory of Pathology Anatomy, Faculty of Veterinary Medicine, Universitas Brawijaya, Universitas Brawijaya, Indonesia; 5Laboratory of Pharmacology, Faculty of Veterinary Medicine, Universitas Brawijaya.

Abstract | Coffee contains high phenols, especially chlorogenic acid which acts as a modulation of the immune response, and antioxidants. Robusta coffee (Coffea canephora) is a type of coffee with high levels of chlorogenic acid and high caffeine content which has the potential as an antioxidant so that it can be used as a green coffee extract (GCE). Green coffee extract is made from roasted coffee beans. This study used 48 layer chickens, consisting of 4 treatments and 12 replications consisting of negative controls (healthy chickens without coffee supplementation), T1 (coffee extract dose 500 mg / kg BW), T2 (coffee extract dose 1000 mg / kg BW) and T3 (coffee extract dose 1500 mg / kg body weight. Data in the form of quantitative duodenum organ histopathology using haematoxylin and eosin staining and histopathological picture of duodenum were observed using a microscope and analyzed descriptively, test for Nrf2 and HO-1 with flow cytometri the result of robusta coffee (Coffea canephora) extract from lampung had a good effect at a dose of 500 mg / kg body weight against the relative number of Nrf2, HO-1, and histopathological improvement in the duodenum. The conclusion of this study shows that robusta coffe e (Coffea canephora) extract has the potential as an anti-inflammatory and antioxidant in chickens.

Keywords | Robusta coffee, Intestine, Antioxidant, Green coffee, Inflammation